Tetanus is a illness caused by a toxin secreted from a bacteria called Clostrium Tetani. Only one type of toxin is produced an it acts on the SNARE synaptobrevin. It cleaves the SNARE so that vesicles containing neurotransmitter cannot fuse with synapse membrane.

Botulism is an illness caused by a toxin secreted from the bacteria C. botulium, like clostridium tetani that causes tetanus it is a member of the clostrium genus of bacteria. Seven different types of botulism toxin can cause botulism all of the toxins act on SNAREs at the synapse that include synaptobrevin, SNAP and syntaxin.

SNAREs are proteins directly involved in membrane fusion and are part of the fusion machinery, SNARE stands for SNAP receptor these proteins bind to SNAPS. There are over 100 different SNARES identified so far and some have specific localaions others can be found in one location only.

SNARE proteins enable vesicles to fuse to their target with a degree of specificity.

Rabs are a type of protein, they are monomeric GTPases that ensure specifcity of vesicular transport in conjunction with SNAREs.

This is a type of chromatography that purifies a protein of interest from a mix of proteins by having ligands for the protein of interest attached to the matrix it is passed through. In this way the protein of interest remains on the matrix and all other proteins from the mix can be washed away.

This is a type of electophroresis that can be used to seperate proteins from a eludate from affinity chromatography. The SDS is a strong detergent that adds a negative charge to the protein so it moves through the gel towards the  positive teminal. The proteins become seperated based on how many negative charges it is labelled with from the SDS, the rate at which they migrate decreases with the number of  negative charges on the protein.